Fig. 1
From: EphrinB2 sharpens lateral motor column division in the developing spinal cord
Expression pattern of ephrinB2 in the ventral neural tube. a/ Schematic representation of transverse sections of mouse embryo at brachial level, with a focus on the LMC in the ventral horn. b/ Detection of EfnB2 mRNA expression (a), ephrinB2 protein expression (b) and H2BGFP expression driven from the endogenous EfnB2 promoter (c) in the ventral neural tube of E12.5 embryos at brachial level. Arrowheads indicate the position of the LMC. C/ Transverse vibratome sections of E11.5 (a-e), E12.5 (f-j), E13.5 (k-o) EfnB2 +/GFP embryos were immunostained for Foxp1 (c, h, m; red) and Islet1 (d, i, n; blue) labeling LMC and LMCm neurons respectively. Epifluorescence of GFP (a, f, k; green) is also represented on heat maps to visualize cells expressing high levels of ephrinB2 (GFPhigh in red). Dotted lines mark Foxp1+/GFPhigh cells. Proportion of Foxp1+/GFPhigh MN expressing Islet1 (LMCm, blue) or not (LMCl, red) in E11.5, E12.5 and E13.5 embryos (n = 3 for each stage) (p). Error bars indicate s.e.m.; Scale bars: 50 μm