Fig. 2
From: The LRR receptor Islr2 is required for retinal axon routing at the vertebrate optic chiasm
Chronological progression of the islr2−/− phenotype. Anterogradely-labeled retinal projections of islr2 mutant embryos and larvae compared to control siblings. At 40 and 48 hpf growth cones can be observed in the proximity of the midline in mutant embryos, while these seem to have proceeded to the optic tract in wild-type siblings. Additionally, axonal extension defects can be recognized in mutants compared to controls (40 hpf, 48 hpf, 72 hpf, islr2−/−, first column). From 48 hpf, ipsilateral neurites (arrow) are visible in mutant embryos and can be traced back to the chiasm. As expected, 5 dpf mutant larvae displayed ectopic innervation of the ipsilateral optic tectum (asterisks). At this stage, islr2−/− larvae show misrouted fibers at the chiasm (arrowheads). All pictures are maximum intensity projections of confocal Z-stacks. Anterior is to the top. Scale bar: 50 μm