Fig. 5

Axon tract and peripheral nerve defects in Wnt1-Cre;Rac1 ^CKO/− embryos. a–g’ Neurofilament (NF) immunostaining of whole-mount E11.5 embryos shows that in Wnt1-Cre;Rac1 ^CKO/− embryos, multiple axon tracts are aberrant including the following: diffuse axonal projections in the hindbrain and the IIIrd and IVth nerves (green arrows in (b) vs. red arrows in (b’)), the Vth nerve (green arrows in (c) vs. red arrows in (c’)), the Xth (green arrow in (d) vs. red arrow in (d’)) and XIIth nerves (green arrowhead in (d) vs. red arrowhead in (d’)), multiple nerves in the forelimb (green arrows in (e) vs. red arrows in (e’)), and ventral axons from the DRG (green arrows in (f) vs. red arrows in (f’)). The sympathetic chain ganglia are reduced in size (green arrows in (g) vs. red arrows in (g’)). To show the individual hindbrain axons, the neurofilament signal intensity was increased in the (b) and (b’) images. d–e’, g, g’ are maximum intensity projections of a subset of Z stacks containing the entire nerves or chain ganglia. N = 3 for each genotype. Scale bars: a’, 1 mm; b’–g’, 100 μm