Fig. 6

RBM8a overexpression prevents cell cycle exit and neuronal differentiation in utero. a Control or RBM8a overexpressing constructs were electroporated into E14 embryonic brains and BrdU was injected at E15. Mice were sacrificed at E16. The cell cycle exit index is calculated as the percentage of the GFP-positive cells that exited the cell cycle (GFP+ BrdU+ Ki67-) divided by total GFP and BrdU double positive (GFP+ BrdU+) cells. n = 3-4, *, p < 0.05, t-test. Scale bar = 10 μm. White arrows indicate GFP + BrdU + Ki67+ cells. Arrowheads indicate GFP + BrdU + Ki67- cells. b Control or RBM8a overexpressing constructs were electroporated into E14 embryonic brains and mice were sacrificed at E16. E16 brain section were stained with neuronal marker Tuj1 (red) and GFP (green). Graphs show the percentage of GFP/Tuj1 double positive cells in each condition. ***, p < 0.001; n = 3–4. t-test. Scale bar = 10 μm