Fig. 6

Both WT and 6T/S-A NeuroD4 generate predominantly sensory neurons (a-e). Embryos were unilaterally injected at the two-cell stage with 100 pg of either WT or 6T/S-A NeuroD4 mRNA and at stage 18, embryos were assayed by qRT-PCR for expression of pan neuronal gene neural-β-tubulin, sensory neuron marker xHox11L2, motor neuron marker xHb9 or interneuron marker xVsx1 (a, b). Data is shown on two separate graphs due to differences in scale [n = 3]. Whole embryo ISH was also conducted to compare the pattern of expression of neural-β-tubulin and xHox11L2 (c) [n = 30–38]. Views in c are all dorso-ventral with injected side to the right; (p < 0.05) = *; (p < 0.025) = **; (p < 0.0125) = ***