Fig. 5

Cumulative mutation of phosphorylation sites creates step-wise increases in proneural activity (a-d). a Schematic representation of the phospho-mutant series of constructs demonstrating the SP and/or TP sites that are mutated to AP sites in each. The series consists of cumulative mutation of sites, working from N to C termini. Two-cell stage embryos were unilaterally injected with 100 pg mRNA of the respective NeuroD4 construct and assayed at stage 18 for expression of neural-β-tubulin by qRT-PCR (B[n = 3]), or whole embryo ISH (C[n = 59-77]) with representative images shown in (d). Views are dorso-ventral with injected side to the right; (p < 0.05) = *; (p < 0.025) = **; (p < 0.0125) = ***