Fig. 3

Similar levels of proneural activity are seen amongst single site mutants (a-d). a Schematic representation of the single site phospho-mutant NeuroD4 constructs demonstrating the relative position of the SP or TP site mutated to AP in each. Two-cell stage embryos were unilaterally injected with 100 pg mRNA of the respective NeuroD4 construct and assayed at stage 18 for expression of neural-β-tubulin by qRT-PCR (B[n = 3]), or whole embryo ISH (C[n = 51-77]) with representative images shown in (d). Views are dorso-ventral with injected side to the right; (p < 0.05) = *; (p < 0.025) = **; (p < 0.0125) = ***