Fig. 1

NeuroD4 is expressed and phosphorylated during primary neurogenesis (A-C). a Protein sequence alignment for human, mouse and Xenopus NeuroD4 homologues using Clustal W. The conserved bHLH domain is indicated in green and SP/TP sites are highlighted in red. A consensus line is also shown below the alignment to indicate the degree of conservation of amino acids at each position: (*) denotes identical residues in all three sequences; (:) denotes highly conserved residues; (.) denotes weakly conserved residues. b Endogenous expression of NeuroD4 at stage 14 (i) and neural-β-tubulin at stage 17 (ii) was determined by whole mount ISH (white arrows correspond to zones of primary neurogenesis; TG = trigeminal ganglia). Dorso-ventral views, anterior up, stages as indicated. c Western Blot analysis of extracts from stage 13 embryos injected with an HA-tagged wild type (WT) NeuroD4 mRNA, either with or without protein phosphatase treatment. Tubulin was used as a loading control