Fig. 9
From: Netrin-1 directs dendritic growth and connectivity of vertebrate central neurons in vivo
Postsynaptic cluster addition and stabilization are modulated by alterations in netrin signaling. a, b Effects of netrin-1, UNC5H2-Ig, or anti-DCC treatments on postsynaptic cluster remodeling were quantified as the proportion of PSD95-GFP puncta that were added (a) and remained stable (b) within the 0–2 and 2–4 observation intervals. Note that significantly more PSD95-GFP puncta were between 0 and 2 h (a), while fewer were stable between 2 and 4 h (b) following netrin-1 or anti-DCC treatment when compared to controls. c To determine the relative stability of newly added postsynaptic clusters, we quantified relative proportion of PSD95-GFP puncta added over the 0- to 2-h interval that were lost in the subsequent 2- to 4-h interval for a subset of randomly selected neurons for each group (n = 4). PSD95-GFP puncta added from 0 to 2 h were significantly less stable in the netrin-1- or anti-DCC-treated neurons. Statistical significance was by one-way ANOVA and with unpaired t-tests. Significance when compared to control is *p < 0.05, **p < 0.01. Error bars indicate SEM