Fig. 7
From: Netrin-1 directs dendritic growth and connectivity of vertebrate central neurons in vivo
Acute manipulations in endogenous netrin levels induce rapid changes in dendrite remodeling. a, b Effects of netrin-1, UNC5H2-Ig, or anti-DCC treatments on new branch addition (a) and branch stabilization (b). Note that while netrin-1 and UNC5H2-Ig increased branch addition and decreased branch stabilization throughout the 24-h imaging period, the anti-DCC treatment influenced the stability of branches at the 4- to 24-h interval only. c Relative proportion of neurons with different branch addition rates. A significant shift in the distribution of neurons that responded with increased branch addition rates was observed after netrin-1 and UNC5H2-Ig treatments. Values are expressed as percent change from total branches. d Relative change in DCI values is shown for each group at all imaging intervals. Note that neurons in UNC5H2-Ig-treated tadpoles significantly decreased their complexity by 4 h compared to controls. Two-way ANOVA with Bonferroni multiple comparison test; *p < 0.05, **p < 0.01, ***p < 0.001. Error bars indicate SEM