Fig. 5
From: Netrin-1 directs dendritic growth and connectivity of vertebrate central neurons in vivo
Altering endogenous netrin levels decreases dendrite branch number and total dendritic arbor length. Effects of tectal microinjection of netrin, UNC5H2-Ig, or netrin + UNC5H2-Ig on total dendrite branch number (a) and length (b). Netrin-1 and UNC5H2-Ig altered tectal neuron morphology with a different time scale. Note that exogenous netrin-1 treatment decreased dendrite arbor length at 24 h, while the UNC5H2-Ig treatment that sequesters endogenous netrin induced a transient but significant decrease in branch number at the 0- to 2- and 0- to 4-h imaging intervals when compared to all other treatments. Co-treatment with netrin + UNC5H2-Ig did not influence branch number or length. Values are expressed as percent change from the initial 0-h imaging session. Two-way ANOVA with Bonferroni multiple comparison test; *p < 0.05, **p < 0.01. Error bars indicate SEM