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Fig. 13 | Neural Development

Fig. 13

From: Netrin-1 directs dendritic growth and connectivity of vertebrate central neurons in vivo

Fig. 13

Sequestration of endogenous netrin-1 with UNC-5 ectodomain affects swimming behavior in a visually guided task. a Schematic of the visual avoidance task viewed from above. Stage 45 tadpoles swim in the 60-mm open field (blue arrow and dotted line) while the Matlab program projects an image on the monitor where the petri dish rests. The black line outside the field represents the vector the 0.3-mm dot (small black circle) will travel. Every 30 s, the 0.3-mm dot appears in the center and is directed towards the black line to intercept the tadpole (black arrow). The tadpole’s response to the advancing stimuli (gray circle) is video recorded and typically results in the tadpole changing its swimming velocity and/or direction (red arrows). b Reaction to the presentation of a moving visual stimulus for tadpoles before treatment (0 h) and 4 h after treatment with vehicle solution (control), netrin-1, anti-DCC, or UNC5H2-Ig is shown as the percent of trials in which tadpoles showed an avoidance response. Tadpoles injected with UNC5H2-Ig had decreased avoidance responses to the presentation of the stimulus 4 h post-injection. Two-way, repeated measures ANOVA with Bonferroni multiple comparison test; *p < 0.05, **p < 0.01. Error bars indicate SEM

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