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Fig. 10 | Neural Development

Fig. 10

From: Netrin-1 directs dendritic growth and connectivity of vertebrate central neurons in vivo

Fig. 10

Overlays of sample neurons at 0, 2, and 4 h illustrate changes in dendritic arbor morphology in response to treatment and between imaging intervals. a Confocal stacks of individual neurons from control, netrin-1-, UNC5H2-Ig-, and anti-DCC-treated tadpoles were reconstructed with MetaMorph creating three-dimensional wireframes of each stack. Wireframes were color-coded based on imaging time point (black, 0 h; blue, 2 h; red, 4 h), overlapped, and aligned over Scholl concentric circles with the primary dendrite placed at a 0° angle (X-axis; gray line). Dynamic changes in dendritic morphology every 2 h over a 4-h imaging period are illustrated by the emergence of blue (2 h) or red branches (4 h) from under the black wireframe (0 h). b, c Cumulative wireframes from a subset of seven neurons per condition better illustrate the dynamic changes in growth between the 0- and 2-h imaging interval (b), and the 0- and 4-h imaging interval (c), for each treatment group. Large arrows point to sample ectopic branches newly extended at the time point indicated by the color of the arrow (blue, 2 h; red, 4 h). Short arrows point to already established branches that changed their directionality of growth at the time point indicated by the color of the arrow (blue, 2 h; red, 4 h)

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