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Figure 6 | Neural Development

Figure 6

From: The methyl binding domain 3/nucleosome remodelling and deacetylase complex regulates neural cell fate determination and terminal differentiation in the cerebral cortex

Figure 6

Altered neuron production and cortical layering in Mbd3-deficient brains. Representative immunostaining of coronal brain sections at E14.5 (A), E16.5 (C) and E18.5 (E) for TBR1 (blue, layer 6), BCL11B (green, layer 5) and SATB2 (red, layers 2 to 4). Quantification of the number of stained cells per 100 μm cortical length at E14.5 (B), E16.5 (D, *P = 0.0040, df = 2 [TBR1]; P = 0.0220, df = 2 [BCL11b]; P = 0.0010, df = 2 [SATB2]) and E18.5 (F, *P = 0.0100, df = 2 [BCL11b]; P = 0.0090, df = 2 [BRN2]). Scale bar = 100 μm; N = 3 to 6. Error bars represent st. dev. White arrows in (B) and (C) point to the BCL11B high and low expressing cells in WT brains, while in cKO brains, only the BCL11B high-expressing cells are present. Red, green and blue bars in (C) and (E), WT merged images indicate distinct layers of cortical neurons present based on the staining. These layers do not appear or are reduced in the Mbd3 cKO brains (yellow bars, (C) and (E) merged images).

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