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Figure 3 | Neural Development

Figure 3

From: The methyl binding domain 3/nucleosome remodelling and deacetylase complex regulates neural cell fate determination and terminal differentiation in the cerebral cortex

Figure 3

Mbd3cKO mice produce fewer differentiated cells. (A) Pregnant mice were injected with a single pulse of BrdU 24 h prior to examination of the embryos at the indicated time points. Differentiated cell output was defined as the number of strongly BrdU (++)/PAX6− cells outside the VZ produced in that 24-h period. Representative immunostaining of E14.5 and E16.5 coronal brain sections for BrdU (red) and PAX6 (green). The white line represents the presumptive boundary between the VZ and outside the VZ (non-VZ). The bottom panels are a magnified image of the boxed area in the above picture. The green, red and yellow arrows point out a PAX6 only +, strong BrdU only + and PAX6/BrdU double-positive cell, respectively. (B) Quantification of the number of differentiated neurons produced in 24 h. Scale bar = 100 μm; N = 3 to 6, *P = 0.0134, df = 2. Error bars represent st. dev. Scale bar = 100 μm.

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Neural Development

ISSN: 1749-8104