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Table 1 Generation of chimeras by morula aggregation

From: Genetic chimeras reveal the autonomy requirements for Vsx2 in embryonic retinal progenitor cells

Aggregation type

Number of morula aggregations attempted

Number of embryos

Implanted a

Recovered

Evaluated for chimerism

Identified as chimeric b

1↔1 aggregations

    

  Vsx2 wt↔Yfp +

58

44

8c

5

3

  Vsx2 orJ↔Yfp +

77

60

19d

13

11

2↔1 aggregations

    

  Vsx2 wt↔Yfp +

120

131e

23f

21

11

  Vsx2 orJ↔Yfp +

128

128g

13h

12

10

  1. aAll successfully aggregated and partially aggregated (successful aggregation of 2 of the 3 morulae during 2↔1 morula aggregations) embryos were implanted into pseudopregnant females; bchimeras identified by evaluation of Yfp + contribution in eye or limb sections; c3 embryos exhibited light or absent eye pigmentation and were excluded from further analysis; d5 embryos exhibited light or absent eye pigmentation and were excluded from further analysis; eincludes 102 successfully aggregated embryos, 18 partially aggregated embryos, and 11 nonaggregated embryos implanted as fillers; f1 embryo was grossly underdeveloped and 1 embryo lacked eye pigmentation. Both embryos were excluded from further analysis; gincludes 112 successfully aggregated embryos and 16 partially aggregated embryos; h1 embryo exhibited abnormal gross morphology and was excluded from further analysis.