Figure 7

Vsx2 ^orJ cells fail to express markers of postmitotic precursors at E12.5. Expression of the combination marker stain (A-D), OTX (E-H), and TUBB3 (I-L) in retinas of wild type (A, E, I), orJ (B, F, J), control chimeras (C, G, K), and mutant chimeras (D, H, L) at E12.5. (Insets) Boxed areas in C, D, G, H, K, and L. Combo stain represents simultaneous staining for ISL1, POU4F, PTF1A, and BHLHB5. The OTX antibody recognizes both OTX1 and OTX2, but the strong, scattered OTX2 expression in postmitotic precursors is readily distinguished from the more peripheral OTX1 upregulation that occurs in the orJ retina (E, F). Dashed lines in I and J delineate the boundary of retinal tissue. All images are maximum Z-projections of confocal scans. Scale bars: 100 μm (A, C, E, G, I, K); 40 μm (insets). Abbreviations: EYFP, enhanced yellow fluorescent protein.