Figure 5

Vsx2 ^orJ cells are rarely located in the differentiated cell layer of chimeras at E12.5. (A) Diagram illustrating the relationship between differentiation status and apical-basal position within the retina. Nascent postmitotic cells (sparse gray cells at apical surface) migrate basally to establish a distinct differentiated cell layer composed of postmitotic neurons. (B, C) Distribution of EYFP+ and EYFP− cells in E12.5 retinas of mutant (B) and control (C) chimeras. (B-inset, C-inset) Boxed areas in B and C. The nuclear marker TOPRO3 demonstrates that the EYFP− regions are not holes, but Vsx2 ^orJ (B-inset) or Vsx2 ^wt (C-inset) cells. Scale bars: 100 μm (B, C); 40 μm (insets). Abbreviations: DCL, differentiated cell layer; EYFP, enhanced yellow fluorescent protein; NBL, neuroblast layer.