Figure 4

Cell-nonautonomous regulation of RPC proliferation by Vsx2. (A, B) EdU incorporation in E12.5 retinas of wild-type and orJ mice. Dashed lines demarcate the neural retina. (C, D) EYFP signal and EdU incorporation in retinas of mutant chimeras in peripheral (C) and central (D) regions. White arrows indicate EdU+ Vsx2 ^orJ (EYFP−) cells in mutant chimeras. Red arrowheads in C demarcate the limits of the peripheral retinal region bounded by the adjacent corneal tissue (left arrowhead) and the intermediate retinal region (right arrowhead). (E) Quantification of EdU+ Vsx2 ^orJ and Vsx2 ^wt cells at E12.5 in peripheral (a), intermediate (b), and central (c) regions of retinas from orJ, wild type, and chimeras. P values calculated by Tukey-Kramer HSD comparison of pairs within, but not across, regions. (F) Quantification of EdU+ Yfp ⁺ cells at E12.5 in peripheral, intermediate, and central regions of retinas from mutant and control chimeras. P values calculated using Student’s unpaired t-test or Welch’s two-sample t-test, as appropriate (based on results of an F-test of variances) within, but not across, regions. Bars represent mean ± standard deviation. *P < 0.04, **P < 0.006, ***P < 0.0001. Nonsignificant changes (all P > 0.2) are not shown. Scale bars: 100 μm. Abbreviations: EYFP, enhanced yellow fluorescent protein.