Figure 2

Comparison of chimerism in control and mutant chimeras across tissues. Schematic diagrams illustrating the tissue organization of the retina (A), cortical epithelium (E), nasal epithelium (I), and limb (M) at E15.5. Endogenous EYFP signal in the retina (B-D), cortical epithelium (F-H), nasal epithelium (J-L), and limb tissues (N-P) identifies cells contributed by the Yfp strain in control and mutant chimeras. Insets in E-H show enlarged region of the ganglionic eminence (inset orientation reflected along the vertical axis and rotated). Control chimera in B, F, J, and N exhibits low Yfp contribution in all tissues examined, while the control chimera in C, G, K, and O exhibits medium levels, and the mutant chimera in D, H, L, and P exhibits high levels of Yfp contribution across tissues. Dashed lines demarcate tissue boundaries. Scale bars: 200 μm. Abbreviations: b, cartilage primordium of turbinate bone (I) or phalangeal/metacarpal bones (M); ge, ganglionic eminence (striatum); iz, intermediate zone of telencephalon; L, lens; lv, anterior horn of lateral ventricle; nc, nasal cavity; np, nasopharynx; npc, neopallial cortex; nr, neural retina; ns, cartilage primordium of nasal septum; oep; olfactory epithelium; onh, optic nerve head; v, vitreous; vz, ventricular zone of telencephalon.