Figure 4

mRNA stability correlates with function and predicted localization. (A) mRNA half-life distributions of all neural mRNAs, the neural fate transcription factor subset (‘neural fate’), and the predicted axon growth cone or dendrite-localized mRNA subset (‘localized’). The horizontal line marks the mean neural-specific half-life (76 min). P values are from Mann-Whitney U tests comparing the distribution of half-lives for each subset to the ‘all mRNAs’ set of transcripts. (B) Representative decay curves for neural fate mRNAs. (C) Representative decay curves for predicted localized mRNAs. For the graphs in B and C, chase times are plotted on the x-axis (0-hour = end of 1-hour pulse). Mean and standard deviation chase/pulse values at the 1-hour chase and 3-hour chase time points are plotted based on multiple measurements across biological replicate experiments.