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Figure 4 | Neural Development

Figure 4

From: Dorsoventral patterning of the Xenopus eye involves differential temporal changes in the response of optic stalk and retinal progenitors to Hh signalling

Figure 4

Overexpression of VP16-Gli1-GR causes stage dependent effects on eye DV polarity. (A) Lateral views of heads of st. 33 embryos that were unilaterally injected with 250 pg of VP16-Gli1-GR mRNA at the eight-cell stage, treated with dex from the indicated stages and hybridized with probes for Pax2, Vax1b, Vax2 and Tbx3. Compared to the control side (uninj.), stage-dependent alterations in gene expression domains are detectable on the injected side. Light-blue β-gal staining identifies the injected side. Scale bar, 200 μm. (B) Quantification of the percentages of embryos with different effects on gene expression domains or eye reductions (S) in each treatment condition. The number of experiments performed for each probe and treatment condition is indicated on top of the corresponding histogram bar. (C) Histological sections of eyes of st. 33 embryos treated as in (A) and (B), and hybridized with the indicated probes, confirming stage dependent gene expression changes as detected in whole mount views. Scale bar, 100 μm.

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Neural Development

ISSN: 1749-8104