Figure 3

Medially shifted somatosensory and visual areas and a reduction in primary visual area in developing BTBR mice. Tangential sections through layer 4 of flattened cortices stained for anti v-Glut2 were obtained from P7 (A, B), P10 (D, E) and P22 (F, G) C57Bl/6 (A, D, F) and BTBR (B, E, G) mice (n  ≥ 6 for each age and strain). V-Glut2-positive PMBSF barrels in S1 at P7, P10 and P22 are indicated with blue arrows, and the v-Glut2-positive V1 is indicated by magenta arrows; the rhinal fissure (demarcating the border between neocortex and olfactory cortex) is indicated by black arrows. (C, H) Schematics of P7 (C) or P10 and P22 tangential sections (H) illustrate the cortical arealisation parameters that were measured in BTBR and C57Bl/6 mice. (I) Quantification of the cortical arealisation parameters in both strains, including total cortical length, width and area; total PMBSF area; PMBSF area normalised to total cortical area; PMBSF position relative to the anterior extent of the cortex; PMBSF position relative to the medial extent of the cortex; total V1 area; V1 area normalised to total cortical area; V1 position relative to the anterior extent of the cortex; and V1 position relative to the medial extent of the cortex. Measurements relating to V1 arealisation were only obtained at P10 and P22, as the V1 area is indistinct at P7 in tangential sections. Measurements were averaged for each strain and displayed as the percentage difference between BTBR and C57Bl/6 (100%). Orientations of tangential sections are indicated in the upper right corner of (H): anterior/rostral to the top, posterior/caudal to the bottom, medial to the left and lateral to the right. Scale bar = 1 mm, BTBR = BTBR T + tf/J, PMBSF = posterior medial barrel subfield.