Skip to main content
Figure 6 | Neural Development

Figure 6

From: Bacurd2 is a novel interacting partner to Rnd2 which controls radial migration within the developing mammalian cerebral cortex

Figure 6

The effect of forced expression of Bacurd2 and its mutated variants on cell migration within the embryonic E17.5 cortex. (A) Coronal sections of E17.5 embryonic cortex following E14.5 in utero electroporation with a bicistronic construct encoding GFP vector only (control), or together with Bacurd2 or its mutant variants. (B) Forced expression of Bacurd2 disrupts the migration of GFP-labelled cells in the embryonic cortex, when compared with control treatment. On the other hand, forced expression of the N-terminal mutants Bacurd2(Δ1-109), Bacurd2(3A) or the C-terminal Bacurd2(Δ221-316) variant did not significantly disrupt the migration of cells (N > 4,000 cells counted from four to six brains per condition. Distribution of GFP-labelled cells within the VZ/SVZ, IZ and CP of the E17.5 cortex; F 8,96 = 5.38; two-way ANOVA followed by Bonferroni’s post hoc test which compares each column to control; *P < 0.05; ***P < 0.001). Scale bar, 100 μm.

Back to article page

Neural Development

ISSN: 1749-8104