Figure 5From: Bacurd2 is a novel interacting partner to Rnd2 which controls radial migration within the developing mammalian cerebral cortex Bacurd2 interacts with its binding partners through distinct regions of the polypeptide. (A) Schematic representation of Bacurd2 polypeptide and its mutant variants used in our analysis. (B) Reciprocal co-immunoprecipitation assays show that Rnd2 interacts with an N-terminal Bacurd2 truncation mutant but is unable to bind the C-terminal truncation mutant Bacurd2(Δ221-316). (C) Cul3 binds Bacurd2(Δ221-316) but not the N-terminal truncation mutant Bacurd2(Δ1-109). (D) Mutations to I71/L72/I73 in the Bacurd2(3A) polypeptide sequence disrupt its interaction with Cul3. (E) Diagrammatic summary of the interactions between Bacurd2 and its interaction partners Rnd2 and Cul3.Back to article page