Figure 2From: Bacurd2 is a novel interacting partner to Rnd2 which controls radial migration within the developing mammalian cerebral cortex Bacurd2 influences cell migration within the embryonic mouse cerebral cortex. (A) Western blotting with HEK293T cell lysates confirms that FLAG-Bacurd2 expression is suppressed by targeting siRNAs, but not by control (non-targeting) siRNAs. Actin was used as loading control. (B) In utero electroporation was performed on embryonic mouse E14.5 embryos and analysed 3 days later at E17.5. Cortical cells were electroporated with control vector (GFP only), a bicistronic GFP expression construct which also encodes Bacurd2, or Bacurd2 siRNA co-electroporated with GFP vector. (C) Quantification reveals that forced expression of Bacurd2, or treatment with Bacurd2 siRNAs, alters the distribution of cells within the embryonic cortex (N > 4,500 cells from four to six brains per condition; F 4,72 = 14.97; P < 0.0001; two-way ANOVA followed by Bonferroni’s post hoc test; ****P < 0.0001. (D) Quantification of GFP+ cells within the CP (divided into the lower, medial and upper CP) reveals that forced expression of Bacurd2 or knockdown of Bacurd2 disrupts the intracortical distribution of GFP+ cells compared with control (N > 1,500 cells from four to six brains per condition; F 4,72 = 27.89; P < 0.0001; two-way ANOVA followed by Bonferroni’s post hoc test). uCP, mCP and lCP indicate upper, medial and lower cortical plates, respectively. Scale bar represents 100 μm.Back to article page