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Figure 2 | Neural Development

Figure 2

From: Bacurd2 is a novel interacting partner to Rnd2 which controls radial migration within the developing mammalian cerebral cortex

Figure 2

Bacurd2 influences cell migration within the embryonic mouse cerebral cortex. (A) Western blotting with HEK293T cell lysates confirms that FLAG-Bacurd2 expression is suppressed by targeting siRNAs, but not by control (non-targeting) siRNAs. Actin was used as loading control. (B) In utero electroporation was performed on embryonic mouse E14.5 embryos and analysed 3 days later at E17.5. Cortical cells were electroporated with control vector (GFP only), a bicistronic GFP expression construct which also encodes Bacurd2, or Bacurd2 siRNA co-electroporated with GFP vector. (C) Quantification reveals that forced expression of Bacurd2, or treatment with Bacurd2 siRNAs, alters the distribution of cells within the embryonic cortex (N > 4,500 cells from four to six brains per condition; F 4,72 = 14.97; P < 0.0001; two-way ANOVA followed by Bonferroni’s post hoc test; ****P < 0.0001. (D) Quantification of GFP+ cells within the CP (divided into the lower, medial and upper CP) reveals that forced expression of Bacurd2 or knockdown of Bacurd2 disrupts the intracortical distribution of GFP+ cells compared with control (N > 1,500 cells from four to six brains per condition; F 4,72 = 27.89; P < 0.0001; two-way ANOVA followed by Bonferroni’s post hoc test). uCP, mCP and lCP indicate upper, medial and lower cortical plates, respectively. Scale bar represents 100 μm.

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