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Figure 1 | Neural Development

Figure 1

From: Differential requirement of F-actin and microtubule cytoskeleton in cue-induced local protein synthesis in axonal growth cones

Figure 1

The effects of pharmacological inhibitors of actin and microtubule polymerization on retinal growth cone morphology. Embryonic eyes at stage 24/25 were cultured for 24 h, treated with either control medium or pharmacological inhibitors for 5 min, and stained for F-actin (Phalloidin-Alexa488) or β-tubulin. Control retinal growth cones (A) contained an extensive network of actin filaments, which was disrupted upon treatment with (B) cytochalasin D (0.1 μM) and (C) latrunculin B (30 nM). Similarly, control retinal growth axons show microtubules in the growth cone central domain and individual microtubules invading the peripheral domain (F). The dynamic microtubules in the growth cone, but not in the axon shaft, were depolymerized upon treatment with (I) colchicine (12.5 μM) or (J) nocodazole (0.1 μM). Importantly, this treatment paradigm of actin or microtubule inhibitors did not affect the ultrastructure of the other cytoskeletal system (G and H, D and E) and also did not increase growth cone collapse at the concentrations used. Scale bar 10 μm.

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