Figure 8
From: Mesoderm is required for coordinated cell movements within zebrafish neural plate in vivo
Neural cell movements in MZ oep embryos are highly disrupted through neural keel to neural tube stages. (A,B) Selected frames from time-lapse sequences showing normal neurulation in a wild-type embryo and disrupted neurulation in a MZoep embryo from 12 hpf. Cells are labeled with H2B-RFP to reveal movements of nuclei (gray). (C,D) Tracks of individual neural cell nuclei in wild-type and MZoep embryos at hindbrain level. Midline is marked with arrowhead. (E) Trajectory plots of cells in wild-type and MZoep hindbrain primordia. (F) Directionality plots for cells in wild-type and MZoep neural plates. (G,H) Speed and persistence of neural cell movements are disrupted from 12 hpf onwards in MZoep embryos (linear speed μm/min: wild-type cells 0.87 versus MZoep cells 0.56, ***P <0.0001 Student’s t-test; persistence: wild-type cells 0.85 versus MZoep cells 0.63, ***P <0.0002 Student’s t-test). (I,J,K,L) The hindbrain marker krox20 shows that the anterior-posterior pattern in hindbrain region is only mildly disrupted despite abnormal cell movements in MZoep neural primordia. H2B-RFP, histone H2B/red fluorescent protein fusion; hpf, hours post fertilization; MZoep, maternal-zygotic one-eyed pinhead; wt, wild-type.