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Figure 3 | Neural Development

Figure 3

From: LPHN3, a presynaptic adhesion-GPCR implicated in ADHD, regulates the strength of neocortical layer 2/3 synaptic input to layer 5

Figure 3

LPHN3 controls the density of synapses formed by L2/3 axons in L5. (A) Confocal image of GFP and tdTomato in a slice of somatosensory cortex from a P21 mouse electroporated in utero to express H2b-GFP, myrTdTomato, and Synaptophysin-GFP. Scale bar = 100 μm. (B, C) Example confocal images of tdTomato-positive axons in L5 decorated with Syp-GFP puncta (white arrowheads). Scale bar = 5 μm. (D, E) shLPHN3 axons, but not Rescue axons, have fewer Syp-GFP puncta than controls at both P14 (One-way ANOVA, F(2,104) = 4.49, P = 0.01: Control, 2.1 ± 0.2 puncta per 10 μm, n = 37; shLPHN3, 1.4 ± 0.2, n = 32; Rescue, 2.2 ± 0.2, n = 38) and P21 (One-way ANOVA, F(2,117) = 10.13, P <0.0001: Control, 2.0 ± 0.1, n = 40; shLPHN3, 1.0 ± 0.1, n = 37; Rescue, 1.8 ± 0.2, n = 43).

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