LPHN3 does not modulate probability of release at L2/3-L5 synapses. (A) Example average light-evoked NMDAR EPSCs recorded at +40 mV for Control and shLPHN3 electroporation conditions. Black traces are responses to a single light flash (blue arrowhead), blue traces are responses to two flashes at 20 Hz, and red traces are the subtraction. The PPR was then calculated as the ratio of the peak amplitudes of red and black traces. (B) The PPRs of L2/3 inputs did not differ between conditions (t-test, t(68) = 1.896, P = 0.06. Control, 0.54 ± 0.03, n = 36; shLPHN3, 0.62 ± 0.04 n = 34). (C) Example average NMDAR EPSCs of consecutive sets of 25 sweeps recorded in the presence 40 μM MK 801. (D) Plot of normalized NMDAR EPSC amplitude by stimulus number for Control and shLPHN3 inputs. The mean number stimuli for the amplitude to reach 50% of baseline did not differ between Control (98.5 ± 8.4, n = 10) and shLPHN3 (89.6 ± 5.9, n = 11) conditions (t(19) = 0.88, P = 0.39). All descriptive statistics and graphs are mean ± SEM.