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Figure 5 | Neural Development

Figure 5

From: Multiple conserved regulatory domains promote Fezf2 expression in the developing cerebral cortex

Figure 5

Enhancer 1316 is active in deep-layer cortical neurons. (A-D) Immunohistochemical analysis of E11.5 brain sections using antibodies for ß-Gal (A), ß-Gal and PAX6 (B), ß-Gal and SOX2 (C), and ß-Gal and Tuj1 (D). (E, F) X-Gal staining of P0 whole mount brains (E) and brain sections (F). (G-I) Immunohistochemistry using ß-Gal antibody combined with antibodies for SOX2 (G), CTIP2 (H ), and SATB2 (I). Arrows represent co-expression of LacZ and the indicated markers. Arrowheads indicate a lack of co-expression. Scale bars: (A) 200 μm, (D) 50 μm, (E) 250 mm, (F) 500 μm, (I) 100 μm, (I’) 25 μm.

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