Figure 3
From: Multiple conserved regulatory domains promote Fezf2 expression in the developing cerebral cortex
Promoter activity of the 2.7 kb fragment during cortical development. (A) X-Gal staining at E11.5 revealed reporter activity in the cerebral cortex, similar to endogenous Fezf2 expression. (B-D) Immunohistochemistry with ß-Gal antibody combined with SOX2 (B), Tuj1 (C), and TBR1 (D) antibodies. (E) Whole mount X-Gal staining of P0 brains demonstrating expression in the forebrain. (F) X-Gal staining of P0 brain sections. (G-I’) Immunohistochemistry using ß-Gal antibody combined with antibodies for SOX2 (G, G’), CTIP2 (H, H’) and SATB2 (I, I’). Arrows represent co-expression of LacZ and the indicated markers. Arrowheads indicate a lack of co-expression. PP, preplate. Scale bars: (A) 200 μm, (D) 50 μm, (E) 250 mm, (F) 500 μm, (I) 100 μm, (I’) 50 μm.