Figure 3From: STRAD pseudokinases regulate axogenesis and LKB1 stabilitySTRADα and STRADβ are redundant in axogenesis and limiting programmed cell death. Embryonic day 18.5 coronal sections immunolabeled for either corticofugal fibers (Tag1, green) (A-D, A’-D’) or activated caspase3 (Act-Casp3, green) (E-H, E’-H’) and a nuclear marker (DRAQ5, magenta in A-D, blue in E-H). Axons are normal in wild-type, STRADα KO and STRADβ cKO brains, but completely gone in the STRADα/STRADβ double KO brain (A-D). Scale bar = 100 μm. Activated caspase3 is largely absent from wild-type, STRADα KO and STRADβ cKO brain, but drastically increased in STRADα/β double KO cortex (E-H, E’-H’). Scale bar = 100 μm. Higher magnifications of cortical regions of TAG1 immunolabeling and activated caspase 3 are shown in grayscale to the right (A’-D’ and E’-H’).Back to article page