Figure 4

Ato is required for neurite guidance in progeny neurons. (A-F’) Neurite guidance defects are observed in ato mutant in L3 and adult brains. (A,B) Immunostaining of wild-type and ato^wL3 cells bearing GFP (green) driven by IPC-Gal4, UAS-nEGFP. Note misguidance (a) and over fasciculation (b) of the neurites (arrowheads in (B)). (C',D') Mutant phenotypes in adult animals. (C,C') Wild-type cells bearing IPC-Gal4, UAS-nEGFP. (C') Close-up showing the axonal and dendritic projections. (D,D') ato^wmutant. Reduction in Me and Lo complex sizes is clear. (D') Close-up showing the axonal and dendritic projections. Some dendrites have growth and fasciculation defects (arrows). Dotted line in (D) marks the border between the Lo complex and Me. (E,F) ato mutant phenotypes in MARCM clones. (Arrowhead a: guidance defects; arrowhead b: fasciculation defect) (E',F') Close-ups of different samples in the neurite region showing overgrowth defects in mutant MARCM clones (arrow in (F')). Scale bars: A-B = 20 μm, C-D = 50 μm, C'-D' = 25 μm, E,F,E',F' = 20 μm. L3, third instar larvae; Lo, lobula; Me, medulla, WT|, wild type.