Figure 1

Ato is expressed in the IPC. (A,B) Ato is expressed in the IPC (arrow) in L3 (green). Dlg was used to mark all cells (magenta). (C-F) Ato⁺ cells are proliferative. The S phase marker BrdU (red in (C) and (F)) but not the mitosis marker Phospho-Histone-H3 (green in (C) and (E)) is present in Ato⁺ cells (blue in (C) and (D)) in the IPC. (G,H) Ato is not expressed in neurons in the IPC. Ato (magenta) and Elav (green) show no co-localization. (H) High magnification of the region in (G) (single section). Scale bars: A,B = 50 μm, C,F = 20 μm, G = 20 μm, H = 8 μm. IPC, inner proliferation center; L3, third instar larvae.