Figure 9

Myosin ii and F-actin dynamics motor activity are required for JAM-C and Cadm3 adhesion dynamics in migrating neurons. CGNs were transfected with expression vectors encoding JAM-C-pHluorin (A, B) or Cadm3-pHluorin (C, D). Time-lapse imaging was used to monitor cell surface adhesion dynamics in migrating cells. After cells were allowed to migrate for 18 to 50 minutes, 50 μM blebbistatin or 5 μM jasplakinolide were added to the culture and imaging continued for a further 28 minutes. Addition of either drug potently inhibited forward movement, JAM-C dynamics, and Cadm3 adhesions. Scale bar, 10 μm.