Figure 8

JAM-C and Cadm3 adhesions are located in the soma and proximal leading process. (A, D) CGNs were electroporated with expression vectors encoding JAM-C-pHluorin and either RFP-UTRCH or RFP-Lifeact. During early migration, JAM-C cell adhesions are observed in the leading process and then accumulate in the trailing process as the soma translocates past the initial adhesion sites in the leading process. JAM-C adhesions in the leading process appear in regions enriched in stable F-actin (labeled by RFP-UTRCH) and dynamic F-actin (labeled by RFP-Lifeact). (B, E) Adaptive volumetric kymographs of the sequences showing the relation of JAM-C adhesions to actin within the soma and leading or trailing processes for the sequences are shown in panel A or D (somal boundaries, dashed blue line; soma center, solid blue line; RFP-UTRCH or RFP-Lifeact, green). (C, F) Analysis of percentage of adhesion signal in the soma and leading or trailing process in migrating neurons expressing JAM-C (green, leading process; red, soma; blue, trailing process). (G, J) CGNs were electroporated with expression vectors encoding Cadm3-pHluorin and either RFP-UTRCH or RFP-Lifeact. During migration, Cadm3 cell adhesions are observed in the leading process and then accumulate in the trailing process as the soma translocates; adhesions in the leading process appear in regions enriched in stable F-actin (labeled by RFP-UTRCH) and dynamic F-actin (labeled by RFP-Lifeact). (H, K) Adaptive volumetric kymographs of the sequences showing the relation of Cadm3 adhesions to actin within the soma and leading or trailing processes for the sequences are shown in panel A or D (somal boundaries, dashed blue line; soma center, solid blue line; RFP-UTRCH or RFP-Lifeact, green). (I, L) Analysis of percentage of adhesion signal in the soma and leading or trailing process in migrating neurons expressing Cadm3 (green, leading process; red, soma; blue, trailing process). Scale bar, 10 μm.