Figure 8
From: Neuropilins define distinct populations of neural crest cells
Nrp2-expressing NCCs give rise to neurons and glia of the dorsal root ganglia. (A) Whole mount X-gal staining of E11.5 Nrp2-CreERT2/Kikume X R26R embryos injected with tamoxifen (TM) at E9.5 and E10.0 (arrows). Staining was identified in presumptive skeletal muscle, skin, and DRG. (B-C) Longitudinal sections anterior to the hind limbs counterstained with eosin confirmed X-gal staining within sensory neurons and glia of the DRG, skeletal muscle and skin. (D-I) Transverse sections anterior to the hind limbs counterstained with Tuj1 and TH demonstrated X-gal staining within sensory neurons of the DRG (E-F, dashed circle) but not within sympathetic neurons (G-I, dashed circle). da, dorsal aorta; nt, neural tube. Scale bars = A, 500 μm; D & G, 100 μm.