Figure 7

Nrp2-expressing NCCs give rise to neurons and glia of the trigeminal ganglia. (A) Whole mount X-gal staining of E11.5 Nrp2-CreERT2/Kikume X R26R embryos injected with tamoxifen (TM) at E9.5 and E10.0 (arrows). Staining was restricted to the trigeminal ganglia (V) that were derived from the r2 stream of NCCs. Notably, staining was absent from derivatives of the r4 stream of NCCs such as the facio-acoustic ganglia (VII-VIII). (B-C) Longitudinal sections through the head of Nrp2-CreERT2/Kikume X R26R embryos counterstained for X-gal and eosin confirmed that transgene expression was restricted to the trigeminal ganglia (V) and lacking in the facio-acoustic ganglia (VII-VIII). (D-G) Serial sections to that in (B) counterstained for X-gal, Tuj1, and Sox9 confirmed transgene expression within neurons and glia of the trigeminal ganglia (V) and not within the facio-acoustic ganglia (VII-VIII). (D) Bright-field (BF) images of X-gal staining overlaid with Tuj1 and Sox9. (G) BF images were colour-inverted to cyan to promote colocalisation analysis. (Gi-Gi′′′ X-gal-positive descendants of Nrp2-expressing NCCs co-expressed Tuj1 (arrow). (Gii-Gii′′′) X-gal-positive descendants of Nrp2-expressing NCCs co-expressed Sox9 (arrow). Scale bars = A, 500 μm; D, G, 100 μm.