Figure 6
From: Neuropilins define distinct populations of neural crest cells
Generation of Nrp2-CreERT2/Kikume lineage-tracing mice. (A) The Nrp2- CreERT2/Kikume transgenic construct consisted of a BAC (RP24-250G22) encompassing 62 kb upstream of the Nrp2 start codon and 4 kb downsteam of the 3′ UTR. A cassette consisting of the sequences for CreERT2, IRES , and Kikume tagged in-frame to the FLAG tag was inserted in place of the start codon of Nrp2. (B-D) Whole mount X-gal staining of E10.0 Nrp2-CreERT2/Kikume X R26R embryos injected with tamoxifen (TM) at E9.0 and E9.5 (arrows). (B,C) In the head, X-gal staining was present in NCCs condensing into the anlagen of the Vth cranial ganglia (dashed circle) and not the VII-VIIIth cranial ganglia. (B, D) In the trunk, X-gal staining was present within the anterior somite. (E-H) Whole mount embryos were sectioned and counterstained with p75. (E-F) Transverse sections through r2 showed that LacZ expression occurred within NCCs (arrow). (G-H) Longitudinal sections through the trunk show that LacZ expression occurred within migrating trunk NCCs (arrow). e, eye; h, heart; nt, neural tube; ov, otic vesicle; *, collection of embryos. Scale bars = A, 10 kb; B, 500 μm; D, 100 μm.