Figure 9

pLL nerve regeneration in sdf1 mutant larvae. (A) A 3 dpf sdf1 mutant larva (also transgenic for neuroD:EGFP) shows a severely altered pLL due to failures in primordium migration during early development. The nerve is located over the yolk rather than along the horizontal myoseptum. (B) The larva was imaged immediately after neurectomy; the point of neurectomy is indicated by an arrow. Arrowhead points to an aberrant branch of the nerve that migrated towards the ventral yolk. (C) A few hours after neurectomy, the distal nerve has degenerated. (D) Twenty-four hours post neurectomy, the nerve has regenerated and follows exactly the same route as that of the original nerve, including the aberrant branch indicated in B (arrowhead). Scale: A-D: 100 μm.