Figure 4From: Genetic dissection of TrkB activated signalling pathways required for specific aspects of the taste systemMorphology of adult fungiform taste bud and taste receptor analysis. (A) Taste bud volume, 3 to 4Ā months old mice (TrkbP/P 24,290āĀ±ā9,377Ā Ī¼m3 vs. TrkbW/W 22,539āĀ±ā11,319Ā Ī¼m3, Pā=ā0.427); (nā=ā3) all genotypes (taste buds: TrkbP/P nā=ā50; TrkbW/W nā=ā39). Values are meanāĀ±ās.d., P statistic from unpaired Studentās t-tests. (BāE) Images of adult innervated taste buds within fungiform papillae highlighted by double immunostaining with TROMA-1 and TUJ1 antibodies. (F) Volume of taste buds in adult (3Ā months old) TrkbS/S and controls (TrkbS/S 25,526āĀ±ā9,746Ā Ī¼m3 vs. Trkb+/+ 23,849āĀ±ā8,539Ā Ī¼m3, Pā=ā0.533); (nā=ā3) each genotype (taste buds for TrkbS/S nā=ā17; Trkb+/+ nā=ā33). Values are meanāĀ±ās.d., P statistic from unpaired Studentās t-tests. (GāJ) Images of Trkb+/+ and TrkbS/S adult innervated taste buds within fungiform papillae highlighted by double immunostaining with TROMA-1 and TUJ1 antibodies. Scale bar, (E, J) 10Ā Ī¼m. (KāN) qRT-PCR analysis. Expression analysis of TATA-binding protein (Tbp) relative to beta actin (Actb) (left), and Cytokeratin-8 (Krt8) relative to Tbp (right) for TrkbP/P mice (nā=ā3) and respective controls (TrkbW/W, nā=ā3) (K). (L) Fold difference of gene expression of various taste receptors in tongue tissues between TrkbW/W controls and TrkbP/P mutants (nā=ā3 per genotype, values are meanāĀ±ās.e.m., P statistic from unpaired Studentās t-tests). (M) Expression analysis of Tbp relative to Actb (left), and Krt8 relative to Tbp (right) for adult TrkbS/S mice (nā=ā4) and respective controls (Trkb+/+, nā=ā4). (N) Fold difference of gene expression of various taste receptors in tongue tissues between Trkb+/+ controls and TrkbS/S mutants (nā=ā4 per genotype, values are meanāĀ±ās.e.m., P statistic from unpaired Studentās t-tests).Back to article page