Skip to main content
Figure 4 | Neural Development

Figure 4

From: Genetic dissection of TrkB activated signalling pathways required for specific aspects of the taste system

Figure 4

Morphology of adult fungiform taste bud and taste receptor analysis. (A) Taste bud volume, 3 to 4Ā months old mice (TrkbP/P 24,290ā€‰Ā±ā€‰9,377Ā Ī¼m3 vs. TrkbW/W 22,539ā€‰Ā±ā€‰11,319Ā Ī¼m3, Pā€‰=ā€‰0.427); (nā€‰=ā€‰3) all genotypes (taste buds: TrkbP/P nā€‰=ā€‰50; TrkbW/W nā€‰=ā€‰39). Values are meanā€‰Ā±ā€‰s.d., P statistic from unpaired Studentā€™s t-tests. (Bā€“E) Images of adult innervated taste buds within fungiform papillae highlighted by double immunostaining with TROMA-1 and TUJ1 antibodies. (F) Volume of taste buds in adult (3Ā months old) TrkbS/S and controls (TrkbS/S 25,526ā€‰Ā±ā€‰9,746Ā Ī¼m3 vs. Trkb+/+ 23,849ā€‰Ā±ā€‰8,539Ā Ī¼m3, Pā€‰=ā€‰0.533); (nā€‰=ā€‰3) each genotype (taste buds for TrkbS/S nā€‰=ā€‰17; Trkb+/+ nā€‰=ā€‰33). Values are meanā€‰Ā±ā€‰s.d., P statistic from unpaired Studentā€™s t-tests. (Gā€“J) Images of Trkb+/+ and TrkbS/S adult innervated taste buds within fungiform papillae highlighted by double immunostaining with TROMA-1 and TUJ1 antibodies. Scale bar, (E, J) 10Ā Ī¼m. (Kā€“N) qRT-PCR analysis. Expression analysis of TATA-binding protein (Tbp) relative to beta actin (Actb) (left), and Cytokeratin-8 (Krt8) relative to Tbp (right) for TrkbP/P mice (nā€‰=ā€‰3) and respective controls (TrkbW/W, nā€‰=ā€‰3) (K). (L) Fold difference of gene expression of various taste receptors in tongue tissues between TrkbW/W controls and TrkbP/P mutants (nā€‰=ā€‰3 per genotype, values are meanā€‰Ā±ā€‰s.e.m., P statistic from unpaired Studentā€™s t-tests). (M) Expression analysis of Tbp relative to Actb (left), and Krt8 relative to Tbp (right) for adult TrkbS/S mice (nā€‰=ā€‰4) and respective controls (Trkb+/+, nā€‰=ā€‰4). (N) Fold difference of gene expression of various taste receptors in tongue tissues between Trkb+/+ controls and TrkbS/S mutants (nā€‰=ā€‰4 per genotype, values are meanā€‰Ā±ā€‰s.e.m., P statistic from unpaired Studentā€™s t-tests).

Back to article page