Figure 2From: Genetic dissection of TrkB activated signalling pathways required for specific aspects of the taste systemTrkB PLCĪ³-site facilitates taste bud innervation during development. (A) E16.5, all neural buds (TrkbP/P 54āĀ±ā17.4 vs. TrkbW/W 113.3āĀ±ā8.3, P <0.01; TrkbD/D 33.3āĀ±ā5, P <0.001 vs. TrkbW/W; Pā=ā0.119 vs. TrkbP/P); Tip (TrkbP/P 35.3āĀ±ā18.6 vs. TrkbW/W 85.3āĀ±ā5.8, P <0.01; TrkbD/D 31.3āĀ±ā4.2, P <0.001 vs. TrkbW/W, Pā=ā0.734 vs. TrkbP/P), (nā=ā3) all genotypes. (B) E16.5 tongue images (tip) stained with P2X3 and TUJ1 antibodies to visualize geniculate neuron afferents and all neuronal fibres, respectively, as well as neural buds. (C, E) P0, all innervated taste buds (TrkbP/P 163.2āĀ±ā7.8 (nā=ā5) vs. TrkbW/W 122.2āĀ±ā17.1 (nā=ā6), P <0.001; TrkbD/D 38.7āĀ±ā7.57 (nā=ā3), P <0.001 vs. TrkbW/W and TrkbP/P). Tip (TrkbP/P (nā=ā5) 133.6āĀ±ā9.2 vs. TrkbW/W (nā=ā6) 101.3āĀ±ā18.2, P <0.01; TrkbD/D 29.3āĀ±ā7.6 (nā=ā3), P <0.001 vs. TrkbW/W and TrkbP/P). All innervated taste buds (TrkbS/S 56.3āĀ±ā3.5 vs. Trkb+/+ 142āĀ±ā11.5, P <0.001); Tip (TrkbS/S 52.3āĀ±ā3.2 vs. Trkb+/+ 117āĀ±ā10.7, P <0.001), (nā=ā3) both genotypes. (D, F) P0 tongue images (tip) stained with TROMA-I and TUJ1 antibodies to visualize innervations into single taste buds. (G, H) Innervated taste buds at 3Ā months, all (TrkbP/P 108.3āĀ±ā28.5 vs. TrkbW/W 90āĀ±ā13.1, Pā=ā0.368); Tip (TrkbP/P 87āĀ±ā23.9 vs. TrkbW/W 73āĀ±ā13.1, Pā=ā0.423). All taste buds (TrkbS/S 15āĀ±ā1 vs. Trkb+/+ 99.3āĀ±ā12.7, P <0.001); Tip (TrkbS/S 14āĀ±ā1 vs. Trkb+/+ 77.7āĀ±ā11.2, P <0.001). (nā=ā3) all genotypes. P statistic from unpaired Studentās t-tests, meanāĀ±ās.d. Arrowheads indicate neural buds (B), and innervated taste buds (D, F) respectively. Scale bars in B, D, F, 400Ā Ī¼m.Back to article page