Table 2 RNA in situ hybridization and antibody probes
From: Spinal neurons require Islet1 for subtype-specific differentiation of electrical excitability
RNA/antibody | Properties | Source |
|---|---|---|
islet1 | 1,900 bp | Dr Judith Eisen, Institute of Neuroscience, University of Oregon, Eugene, OR, USA |
nrp1a | 1,200 bp | Dr Waturu Shoji, Department of Cell Biology, Institute of Development, Aging and Cancer, Tohoku University, Sendai, Japan |
vglut2.1 | 500 bp | Dr Shin-ichi Higashijima, Okazaki Institute of Integrative Bioscience, Okazaki, Japan |
vglut2.2 | 500 bp | |
gad65 | 900 bp | |
gad67 | 900 bp | |
olig4 | 1000 bp | Dr Francesco Argenton, Department of Biology, University of Padua, Padua, Italy |
scn8aa | 655 bp | Our work |
runx3 | 1,900 bp | Dr Phil Crosier, Dept Mol Medicine, University of Auckland, Auckland, New Zealand |
anti-GFP | Rabbit polyclonal, (1:700) | Molecular Probes-Life Technologies, Grand Island, NY, USA |
anti-zn12 (HNK) | Mouse monoclonal, (1:100) | DSHB* |
anti-GABA | Rabbit polyclonal, (1:250) | Sigma-Aldrich Co., St. Louis, MO, USA |
goat anti-rabbit | Conjugated to Alexa-568/488, (1:1000) | Molecular Probes-Life Technologies |
goat anti-mouse | Conjugated to Alexa-488, (1:1000) | Molecular Probes-Life Technologies |