Figure 1
From: Pioneer midbrain longitudinal axons navigate using a balance of Netrin attraction and Slit repulsion
Netrin1 and deleted in colorectal cancer receptor expression is closely associated with the medial longitudinal fasciculus in the early brain. (A-C) Netrin1 expression pattern revealed by in situ hybridization (ISH) in embryonic day 10.5 mouse embryos. Side view of bisected neural tubes. (A) The location of the nucleus of the medial longitudinal fasciculus (nMLF) is indicated (*). (B) Close up showing axons, as labeled by anti-β-tubulin, with respect to Netrin1 expression. The medial longitudinal fasciculus (MLF) travels through the Netrin1-positive region (arrow). (C) Cartoon showing MLF (red) and Netrin1 expression. Dark blue is strong Netrin1 expression while light blue is weaker Netrin1 expression. (D,E) Deleted in colorectal cancer (DCC) expression pattern, revealed by ISH for mRNA (D) and DCC antibody (Ab) labeling (E). The position of the nMLF is noted (*) in (D), and the MLF bundle (arrow) in (E). Scale bar in (B) indicates 200 μm; scale bar in (E), 200 μm, applies to (A,D,E). mb, midbrain; op, optic vesicle; r1, rhombomere 1.