Table 1 Antibodies used in these studies
Antigen | Isotype | Description of immunogen | Source/Catalog number | References | Dilution for immunohistochemistry |
|---|---|---|---|---|---|
Glutamate Decarboxylase 67 (Gad 67) | Mouse IgG2a | Recombinant Gad67 | Millipore #MAB5406 | 1:500 | |
Glutamate Decarboxylase 65 (Gad 65) | Rabbit IgG | Synthetic peptide containing amino acids 572 to 585 of rat Gad65 | Millipore #AB1511 | 1:500 | |
Vesicular Glutamate Transporter 1 (VGluT1) | Rabbit IgG | Recombinant fusion protein consisting of amino acids 456 to 560 of rat VGlut2 | Synaptic Systems #135303 | 1:500 | |
Vesicular Glutamate Transporter 2 (VGluT2) | Rabbit IgG | Recombinant fusion protein consisting of amino acids 510 to 582 of rat VGlut2 | Synaptic systems #135403 | 1:500 | |
Vesicular GABA Transporter (VGAT) | Rabbit IgG | Synthetic peptide containing amino acids 510 to 525 of rat GABA | Synaptic systems #131103 | 1:500 | |
Vesicular Acetyl-choline Transporter (VAChT) | Guinea Pig IgG | Fusion protein consisting of amino acids 475 to 530 of rat VAChT | Synaptic systems #139105 | [76] | 1:250 |