Figure 6

Fate mapping of dopaminergic neurons, red nucleus neurons, oculomotor neurons, and serotonergic neurons from Sonic hedgehog (Shh)-expressing cells. Using the Shh-Cre;R26R reporter line, we found that (A) tyrosine hydroxylase (TH)+ DA neurons, (D) Brn3a+ neurons in the red nucleus, (G) Isl1+ oculomoter neurons (H) and 5-hydroxytryptamine (5-HT)+ serotonergic neurons showed extensive coexpression of β-galactosidase at embryonic day (E)12.5. (A and D insets) Arrowheads show the colocalization of (A inset) TH (red) and (D inset) Brn3a (red) with β-galactosidase (green) at E12.5. At post-natal day (P)0, (B) TH+, (E) Brn3a+, (H) Isl1+ and (K) 5-HT+ neurons expressed within the LacZ-expressed regions (blue). (C,F,I,L) Higher magnifications of the boxed regions in (B,E,H,K), respectively. Black arrowheads indicate the colocalized cells. Scale bars: (A) 50 μm, applied to A, D, G, E); (B, F, H) 100 μm; (C) 50 μm; (E) 200 μm.