Figure 4
From: Temporal and spatial requirements of Smoothened in ventral midbrain neuronal development
Region-specific removal of Smoothened in the ventral midbrain of Shh-Cre;Smofl/flmutants. (A-F) Using the R26R reporter line, we found that Shh-Cre drives recombination in D1 domain and the majority of D2 domain from embryonic day (E)9.5 to E11.5. (G-I’) In situ hybridization of Smoothened indicates complete removal of Smoothened in Shh-Cre;Smofl/fl mice from E9.5 to E11.5, and (G-G’ and insets) incomplete removal at E8.5 (eight somites). Scale bars: (A) 25 μm, applied to A-B; (C) 50 μm, applied to C-F; (G) 200 μm, applied to G and I’.