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Figure 2 | Neural Development

Figure 2

From: Temporal and spatial requirements of Smoothened in ventral midbrain neuronal development

Figure 2

Dynamic expression patterns of Shh signaling components in the developing ventral midbrain (vMB). (A) Immunohistochemical staining and in situ hybridization (insets) revealed Shh expression in the midline of vMB at embryonic day (E)8.5 (10 somites). (B-C and insets) Shh expression in vMB extended laterally from E9.5 toE10.5, then (D, inset) became restricted laterally at E11.5. (E-H) In situ hybridization for Smoothened from E8 to E11.5 show that Smoothened was diffusely expressed both dorsally and ventrally in midbrain and its expression became progressively restricted to the ventricular zone in vMB at E10.5 to E11.5. (F, G, H) Dashed lines outline the pia side of neurotube. (I-X) In situ hybridization for (I-L) Patched, (M-P) Gli1, (Q-T) Gli2, and (U-X) Gli3 from E8 to E11.5. All were expressed medially at E8 to E8.5 (7–10 somites), and then shifted laterally from E9.5 onwards. Scale bars: (A), 50 μm, applied to A, B; (C) 200 μm, applied to C, D, H; (E) 25 μm, applied to E, I M, Q, U; (F) 200 μm, applied to F, J, N, R, V; and (G) 500 μm, applied to G, K, O, S, W, L, P, T, X.

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