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Figure 7 | Neural Development

Figure 7

From: AKT activation by N-cadherin regulates beta-catenin signaling and neuronal differentiation during cortical development

Figure 7

Dominant negative AKT increases apoptotic cell death in vivo. E13.5 forebrains were electroporated in utero with pCAG-GFP and 4-fold (by mass) excess of pcDNA control or K197M dominant negative AKT (DN-AKT) and analyzed at E14.5. Sections of electroporated brains were stained for GFP, cleaved caspase 3, and DNA stained with DAPI. Arrows point to electroporated cells that are expressing cleaved caspase 3. Bright red round spots are non-specific staining not associated with cells. Bar = 50 µm. Compared with control, electroporation of DN-AKT causes increased cell death, P = 0.0067 by paired students t-test. n = 3 brains for each condition. EP’d, electroporated.

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